R&D CENTER

A Genome Sequence of Clammy Goosefoot, Dysphania pumilio

Yongsung Kim and Jongsun Park*
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The genus Dysphania R.Br. consisting of 7-10 species endemic to Australia belongs to the tribe Dysphanieae in Amaranthaceae. Recent phylogenetic research shows that Dysphania is clearly distinguished from Chenopodium by having the presence of multicellular glandular hairs and closely related to Suckleya and Cycloloma. Dysphania pumilio (R. Br.) Mosyakin & Clemants is the native species to Australia (Scott, 1978; Wilson, 1983; 1984) and it has been spread out to many countries except Africa since 1979. It is strong enough to grow in waste areas or even in tiny space in roadsides. We sequenced genome of Dysphania pumilio, invasive species in Korea, using HiSeq 4000 with four different libraries. Total length of genome is 365.76 Mb (N50 is 81,724bp), covering 80\% of k-mer analysis result (456 Mb) and max length of scaffold is 1.02Mbp. Number of genes predicted by AUGUSTUS is 73,911 among which 43,743 genes (59.18\%) have functional domains detected by InterProScan. Functional domain comparison of Chenopodium quinoa genome of which genus is neighbor to Dysphania and D. pumilio presents that 87.24\% (5,810) functional domains are shared with both genomes, and 333 functional domains (5.00\%) are D. pumilio specific. 47,606 Simple sequence repeats (SSRs) were identified from D. pumilio genome, presenting that proportions of diSSRs (22.68\%) and hexaSSR (4.57\%) are higher than those of C. quinoa (15.02\% and 2.40\%, respectively). D. pumilio genome, which is the first genome in Dysphania genus will provide detailed landscape of Dysphania genomes as well as phylogenetic relationship between Dysphania and Chenopodium.