R&D CENTER

Complete Chloroplast Genome sequence of Salix koriyanagi

Juhyeon Kim, Yongsung Kim and Jongsun Park
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Salix L., which is the largest genus in Salicaceae, contains around 400 to 500 species distributed all over the world. Salix is a genus which is difficult to be correctly identified due to frequent hybridization and lack of identification characters. To understand phylogenetic position of Salix koriyanagi Kimura, which is Korean endemic plant, we sequenced chloroplast (cp) genome using next-generation sequencing platform. The assembled sequence displays 155,771 bp long and has a typical quadripartite structure that contains two single copy regions. a large single copy region (LSC, 84,548 bp), and a small single copy region (SSC, 16,125 bp) separated by inverted repeat regions (IRs, 27,459 bp). Gene annotation revealed that the S. koriyanagi cp genome encoded 130 unique genes, including eight ribosome RNA genes, 38 transfer RNA genes, and 83 protein-coding genes. Together with more Salix cp genome sequences which we plan to sequences in near future, S. koriyanagi cp genome successfully assembled will be a useful resource for understanding phylogenetic relations in Salix genus.