Korean effective microorganisms (KEM) comprise a commercial microbial complex originating from the microorganisms found in traditional Korean fermented foods, including kimchi. Using next-generation sequencing (NGS) technology, the complete genome sequence of Lentilactobacillus parabuchneri, considered a major species of KEM, was assembled. L. parabuchneri strains display large numbers of single nucleotide polymorphisms (SNPs) and indels, including gene deletions. Copyright © 2021 Park et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.