The Dolichoderus quadripunctatus group is a taxonomic group found in the Holarctic regions, characterized by their propodeum overhanging the petiole and pronotum without angles. Currently four North American and two Eurasian species have been recognized. Eurasian species is composed of European D. quadripunctatus and east Asian D. sibiricus , separated by a large non habited central Asian region. Based on the morphological and genetic analyses of the two species, D. sibiricus collected from Jeonju was distinct in both morphological and genetic features: Mitochondrial genomes of the Jeonju isolate and D. quadripunctatus from Poland together with that of D. sibiricus isolated from Seoul represent that D. sibiricus from Seoul was more related to the European D. quadripunctatus than D. sibiricus from Jeomju , confirming the two population as separate species, suggesting the two Palearctic species and a plausible additional species. The two types of D. sibiricus could be separated by the color, ratio of the head and the density of the punctations. Additional phylogenetic tree constructed from COXI sequences shows i ) monophyly of both Paelearctic and Nearctic species of the group and ii) the group’s close relation with South East Asian species suggesting the Asian origin of D. quadripunctatus group and a following dispersal of the group to North America.