Pseudostellaria longipedicellata which belongs to Caryophyllaceae is endemic in South Korea. In this study, we presented first complete chloroplast genome of P. longipedicellata which is 149,626 bp and has four sub-regions: 81,292 bp of large single copy (LSC) and 16,984 bp of small single copy (SSC) regions are separated by 25,675 bp of inverted repeat (IRs) regions including 126 genes (81 CDS, eight rRNAs, and 37 tRNAs). The overall GC contents of the chloroplast genome were 36.5\% and in the LSC, SSC and IR regions were 34.3\%, 29.3\%, and 42.4\%, respectively.